High-throughput sequencing in mitochondrial DNA research

نویسندگان
چکیده

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

High-throughput sequencing technologies.

The human genome sequence has profoundly altered our understanding of biology, human diversity, and disease. The path from the first draft sequence to our nascent era of personal genomes and genomic medicine has been made possible only because of the extraordinary advancements in DNA sequencing technologies over the past 10 years. Here, we discuss commonly used high-throughput sequencing platfo...

متن کامل

DNA Methylation Profiling from High-Throughput Sequencing Data

The methylation of a cytosine at the carbon 5 position (5meC) is a common epigenetic mark in eukaryotic cells that is normally found in CpG and CpHpG (H=A,C,T) sequence contexts. The inactivation of one of the X-chromosomes in female cells, the allele-specific expression of imprinted genes or the role in the embryonic development which is shown by the early lethality of Dnmt3a and Dnmt3b defici...

متن کامل

End-to-End Optimization of High-Throughput DNA Sequencing

At the core of Illumina's high-throughput DNA sequencing platforms lies a biophysical surface process that results in a random geometry of clusters of homogeneous short DNA fragments typically hundreds of base pairs long-bridge amplification. The statistical properties of this random process and the lengths of the fragments are critical as they affect the information that can be subsequently ex...

متن کامل

Simple, robust methods for high-throughput nanoliter-scale DNA sequencing.

We have developed high-throughput DNA sequencing methods that generate high quality data from reactions as small as 400 nL, providing an approximate order of magnitude reduction in reagent use relative to standard protocols. Sequencing of clones from plasmid, fosmid, and BAC libraries yielded read lengths (PHRED20 bases) of 765 +/- 172 (n = 10,272), 621 +/- 201 (n = 1824), and 647 +/- 189 (n = ...

متن کامل

High-Throughput DNA Sequencing Analysis of Antibody Repertoires.

New high-throughput DNA sequencing (HTS) technologies developed in the past decade have begun to be applied to the study of the complex gene rearrangements that encode human antibodies. This article first reviews the genetic features of Ig loci and the HTS technologies that have been applied to human repertoire studies, then discusses key choices for experimental design and data analysis in the...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Mitochondrion

سال: 2014

ISSN: 1567-7249

DOI: 10.1016/j.mito.2014.05.004